根据活细胞能吸收结晶紫染料的原理 ,建立了测定干扰素效价的结晶紫染色法 ,并用线平行线定量法计算干扰素含量。经试验测定 ,该方法与用常规法测得的结果有很好的相关性 (r =0 .9387,P >0 .0 5 ) ,且重复性较好 ,用 A值表示细胞的病变程度比打分法能够更准确地反应细胞的状态 ,因此所得结果更精确、客观 ,表明该方法可用于干扰素类制品的效价测定。 According to the principle that living cells can absorb crystal violet dye, a crystal violet staining method was established to determine the potency of interferon, and the interferon content was calculated by line parallel line quantitative method. The test results show that this method has good correlation with the results obtained by routine method (r = 0. 9387, P> 0.05), and has good repeatability. The value of A indicates the degree of lesion in cells Scoring method can more accurately reflect the state of cells, so the results obtained more accurate and objective, indicating that the method can be used for interferon products titer.