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目的:探讨催产素受体基因敲除对雌激素调节骨密度的影响。方法:选取3月龄野生型Oxtr+/+和纯合型Oxtr-/-雌性BABL/c小鼠各20只,随机分成野生型组(WT组)、野生型+雌二醇组(WT+E2组)、野生型去势组(WTOVX组)、野生型去势+雌二醇组(WTOVX+E2组)、纯合型组(KO组)、纯合型+雌二醇组(KO+E2组)、纯合型去势组(KO OVX组)及纯合型去势+雌二醇组(KO OVX+E2组),每组5只。17β-雌二醇皮下注射,每只50μg.d-1,每天1次。实验4周后测量各组小鼠脊柱和股骨的骨密度。结果:与WT组相比较,WT+E2组的骨密度显著增高(P<0.05),WTOVX组的骨密度显著降低(P<0.05),WTOVX+E2组的骨密度差异无统计学意义(P>0.05);与WTOVX组相比较,WTOVX+E2组的骨密度明显增高,差异具有统计学意义(P<0.05);与KO组相比较,KO+E2组、KO OVX组及KO OVX+E2组的骨密度差异均无统计学意义(P>0.05);KO OVX+E2组与KO OVX组相比较,骨密度差异也无统计学意义(P>0.05)。结论:催产素参与调节雌激素的骨合成代谢作用。
Objective: To investigate the effect of oxytocin receptor gene knockout on estrogen-regulated bone mineral density. Methods: Twenty (20) wild-type Oxtr + / + and homozygous Oxtr - / - female BABL / c mice were randomly divided into three groups: WT group, WT group and WT + E2 group (WTOVX group), wild type castration + estradiol group (WTOVX + E2 group), homozygous group (KO group) and homozygous + estradiol group (KO + E2 (KO OVX group) and homozygous castration + estradiol group (KO OVX + E2 group), with 5 rats in each group. 17 [beta] -estradiol subcutaneously, each 50 [mu] g.d-1 once daily. Four weeks after the experiment, the bone density of the spine and femur in each group was measured. Results: Compared with WT group, BMD of WT + E2 group was significantly increased (P <0.05), BMD of WTOVX group was significantly decreased (P <0.05), and BMOD of WTOVX + E2 group was not significantly different > 0.05). Compared with WTOVX group, the BMD of WTOVX + E2 group was significantly higher than that of WTOVX group (P <0.05). Compared with KO group, KO + E2 group, KO OVX group and KO OVX + E2 (P> 0.05). There was no significant difference in bone mineral density between KO OVX + E2 group and KO OVX group (P> 0.05). Conclusion: Oxytocin is involved in the regulation of estrogen’s bone anabolism.