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目的:探讨子宫颈癌、子宫颈上皮内瘤变组织中高危型HPV(HPV16/18)感染及其与P53、Ki-67表达水平的关系。方法:应用原位杂交技术对正常宫颈组织22例、宫颈上皮内瘤变(CIN)55例、66例宫颈癌组织中HPV16/18感染进行检测,并应用免疫组织化学方法对其上述宫颈组织P53、Ki-67的表达进行检测。结果:HPV16/18在正常宫颈、宫颈上皮内瘤变(CIN)、宫颈鳞癌中检出率分别为13.64%、40.00%、75.76%;P53在3组阳性表达率分别为9.09%、23.64%、53.03%;Ki-67在3组阳性表达率分别为18.18%、34.54%、78.79%;Ki-67增殖指数随CIN级别的升高而明显增加(P<0.01);P53、Ki-67在正常宫颈组织、子宫颈上皮内瘤变和子宫颈癌组织中表达差异有统计学意义(P<0.01、P<0.05)。结论:HPV16/18、P53、Ki-67与子宫颈癌发生均有密切关系,HPV16/18、P53、Ki-67联合检测可用于评价CIN细胞增殖活性及其转归,可以作为子宫颈癌早期诊断及预后评估的临床指标。
Objective: To investigate the relationship between high-risk HPV (HPV16 / 18) infection and the expression of P53 and Ki-67 in cervical cancer and cervical intraepithelial neoplasia. Methods: In situ hybridization was used to detect HPV16 / 18 infection in 22 cases of normal cervical tissue, 55 cases of cervical intraepithelial neoplasia (CIN) and 66 cases of cervical cancer. Immunohistochemistry was used to detect the expression of P53 , Ki-67 expression was detected. Results: The positive rates of HPV16 / 18 in normal cervical and cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma were 13.64%, 40.00% and 75.76%, respectively. The positive rates of P53 in the three groups were 9.09% and 23.64% , And 53.03% respectively. The Ki-67 positive rates in the three groups were 18.18%, 34.54% and 78.79% respectively. Ki-67 proliferation index increased significantly with the increase of CIN level (P <0.01) There was significant difference between normal cervical tissue, cervical intraepithelial neoplasia and cervical cancer (P <0.01, P <0.05). Conclusions: HPV16 / 18, P53 and Ki-67 are closely related to the occurrence of cervical cancer. The combined detection of HPV16 / 18, P53 and Ki-67 can be used to evaluate the proliferation and prognosis of CIN, which can be used as early stage of cervical cancer Diagnosis and prognosis of clinical indicators.