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目的建立稳定、可靠、简便的H PV原位杂交检测方法。方法采用北京中杉金桥生物技术公司的H PV定型(6/11、16/18、31/33)原位杂交试剂盒,利用病理科宫颈癌石蜡组织、妇科宫颈及外阴活检组织经10%甲醛固定,常规石蜡包埋后,用防脱片制成4μm石蜡切片,操作步骤基本按试剂盒说明进行(其中有些改动)。结果D N A阳性定位清晰准确,在上皮组织的挖空细胞核中,且背景透明干净。结论该法操作简便,直观准确,可稳定地用于临床H PV感染的检测,方便了患者,也为临床对症治疗提供了依据。
Objective To establish a stable, reliable and convenient H PV in situ hybridization assay. Methods H PV stereotypes (6/11, 16/18, 31/33) in situ hybridization kits from Beijing Zhongshang Jinqiao Biotechnology Company were used. The paraffin-embedded tissues of cervical cancer, gynecologic cervix and vulvar biopsies were fixed by 10% formalin , Conventional paraffin embedding, with anti-off pieces made of 4μm paraffin sections, the basic steps according to kit instructions (some of these changes). Results D N A positive positioning clear and accurate, hollow nuclei in epithelial tissue, and the background is transparent and clean. Conclusion The method is simple, intuitive and accurate, and can be used stably for the detection of clinical H PV infection, which is convenient for patients and provides the basis for clinical symptomatic treatment.