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目的 探讨幽门螺杆菌 (Hp)感染与APC基因突变的关系。方法 采用改良的Giemsa染色和PCR方法检测Hp;采用DGGE电泳和DNA测序技术检测APC突变。结果 6 8例胃癌中检出APC基因突变 15例 ,突变率为 2 2 .1%。APC突变率在肠型胃癌显著高于弥漫型胃癌 (P <0 .0 5 )。将MSI分为高频率MSI(MSI H ,≥ 2个位点 ) 8例、低频率MSI(MSI L ,仅为 1个位点 ) 9例和MSI阴性 (MSS) 5 1例 3组 ,结果APC突变均发生于MSI L和MSS组 ,而MSI H组未发现有APC突变者。APC基因突变在Hp+组和Hp -组及CagA +组和CagA -组检出率均无显著差异 (P >0 .0 5 )。结论 APC突变参与了LOH途径 ;胃癌粘膜Hp感染与APC基因突变无关
Objective To investigate the relationship between Helicobacter pylori (Hp) infection and APC gene mutation. Methods Hp was detected by modified Giemsa staining and PCR method. APC mutation was detected by DGGE electrophoresis and DNA sequencing. RESULTS: A total of 15 APC mutations were detected in 68 gastric cancers, and the mutation rate was 22.1%. APC mutation rate was significantly higher in intestinal type gastric cancer than in diffuse type gastric cancer (P < 0.05). MSI was divided into high-frequency MSI (MSI H, ≥ 2 sites) in 8 patients, low-frequency MSI (MSI L, only 1 site) in 9 patients, and MSI-negative (MSS) in 51 patients in 3 groups. Results APC The mutations occurred in the MSI L and MSS groups, while no MSP mutations were found in the MSI H group. There was no significant difference in the detection rate of APC gene mutations between the Hp+ and Hp-groups, and the CagA+ and CagA-groups (P > 0.05). Conclusion APC mutations are involved in the LOH pathway; gastric mucosal Hp infection is not associated with APC gene mutations