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本文在银胶活性基质上获得了Cu(Ⅰ)的中位-四(4-N-氰甲基吡啶)卟啉络合物Cu(Ⅰ)NACN的表面增强喇曼光谱(SERS)光谱,并与其RRS光谱作了比较,讨论了pH值和Cu(Ⅰ)NACN的浓度对其SERS光谱的影响,研究了小牛胸腺双链DNA及其变性DNA与Cu(I)NACN的复合物的SERS光谱,指出Cu(Ⅰ)NACN是通过吡啶氮和卟啉核共同与银胶作用的,Cu(Ⅰ)NACN是斜向吸附在银表面上的,最佳pH范围在5～6,最佳浓度在大约10～5mol/L;双链DNA和变性DNA的加入不能改变Cu(Ⅰ)NACN在银胶表面上的斜向吸附,在银胶体系中双链DNA的磷酸根通过静电力与Cu(Ⅰ)NACN起弱相互作用,Cu(Ⅰ)NACN可能部分插入变性DNA单链并与之存在同双链DNA一样的静电作用.
In this paper, the surface enhanced Raman spectroscopy (SERS) spectra of Cu (Ⅰ) NACN (Cu (Ⅰ)) - tetrakis (4-N-cyanopyridine) The effects of pH value and concentration of Cu (Ⅰ) NACN on its SERS spectra were discussed. The SERS spectra of double stranded DNA and its modified DNA with Cu (I) NACN were studied. , Indicating that Cu (Ⅰ) NACN interacts with silver colloid through pyridine nitrogen and porphyrin nucleus. Cu (Ⅰ) NACN is adsorbed diagonally on the surface of silver. The optimum pH range is 5 ~ 6. The optimum concentration of Cu About 10 ~ 5mol / L. Addition of double-stranded DNA and denatured DNA can not change the oblique adsorption of Cu (Ⅰ) NACN on the surface of silver colloids. ) NACN plays a weak interaction, Cu (Ⅰ) NACN may be partially inserted into the denatured DNA single-stranded and with the existence of the same double-stranded DNA electrostatic effect.