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目的探讨中国北方汉族人中HLA-A、B、DRB1等位基因与肺癌遗传易感性间的关系。方法采用测序分型技术(sequence-based typing,SBT)技术,对无血缘关系的籍贯为中国北方的140名肺癌患者及483名健康志愿者的HLA-A、B、DRB1基因进行检测。arlequin软件(ver2.000)及SPSS软件进行统计分析。结果 A*2601、B*1518、B*3802、DRB1*0401、DRB1*0402、DRB1*1201在肺癌病人中频率显著性高于正常对照,P值分别为0.021、0.001、0.015、0.021、0.010和0.046,OR值分别为3.513、3.842、2.715、3.512、13.986、1.828;HLA-DRB1*1001、DRB1*1302在肺癌病人中频率显著性低于正常对照,P值分别为0.017和0.014,OR值分别为0.135和0.122。单倍型HLA-A*0207-B*4601-DRB1*0901、HLA-A*0206-B*5101在肺癌病人中频率显著性高于正常对照,P值分别为0.034和0.006,OR值分别为2.348和3.969;单倍型HLA-A*1101-DRB1*1501在肺癌病人中频率显著性低于正常对照,P值为0.026,OR为0.146,另外单倍型HLA-A*0101-B*3701在正常对照中频率为0.02070但在肺癌病人中未检出。结论在中国北方人中HLA-A*2601、B*1518、B*3802、DRB1*0401、DRB1*0402、DRB1*1201和单倍型HLA-A*0207-B*4601-DRB1*0901、HLA-A*0206-B*5101与肺癌发病正相关,HLA-DRB1*1001、DRB1*1302和单倍型HLA-A*1101-DRB1*1501、HLA-A*0101-B*3701与肺癌发病负相关。
Objective To investigate the relationship between HLA-A, B and DRB1 alleles and genetic susceptibility to lung cancer in Han Chinese from northern China. Methods Sequencing-based typing (SBT) was used to detect HLA-A, B and DRB1 genes in unrelated lung cancer patients from 140 lung cancer patients and 483 healthy volunteers in northern China. arlequin software (ver2.000) and SPSS software for statistical analysis. Results The frequency of A * 2601, B * 1518, B * 3802, DRB1 * 0401, DRB1 * 0402 and DRB1 * 1201 were significantly higher in patients with lung cancer than those in normal controls, with P values of 0.021,0.001,0.015,0.021,0.010 and The OR of HLA-DRB1 * 1001 and DRB1 * 1302 in patients with lung cancer was significantly lower than that of the normal controls (P = 0.017 and 0.014, respectively), and OR values were respectively 3.513, 3.842, 2.715, 3.512, 13.986 and 1.828 0.135 and 0.122. The frequencies of haplotype HLA-A * 0207-B * 4601-DRB1 * 0901 and HLA-A * 0206-B * 5101 in patients with lung cancer were significantly higher than those in normal controls, with P values of 0.034 and 0.006, respectively 2.348 and 3.969 respectively. The frequency of HLA-A * 1101-DRB1 * 1501 haplotype was significantly lower in patients with lung cancer than in normal controls (P = 0.026, OR = 0.146) In normal controls the frequency was 0.02070 but not detected in lung cancer patients. Conclusion HLA-A * 2601, B * 1518, B * 3802, DRB1 * 0401, DRB1 * 0402, DRB1 * 1201 and haplotype HLA- A * 0207- B * 4601- DRB1 * 0901, HLA -A * 0206-B * 5101 was positively correlated with the incidence of lung cancer. HLA-DRB1 * 1001, DRB1 * 1302 and haplotype HLA-A * 1101-DRB1 * 1501 and HLA- Related.