论文部分内容阅读
以仙人掌为原料提取多糖产品,通过超滤浓缩和不同浓度乙醇分级沉淀,分别得到55%乙醇沉淀的多糖(OPMC I),进一步乙醇浓度增至80%沉淀的多糖(OPMCⅡ),以及一次性80%乙醇沉淀的多糖(OPMCⅢ)。比较了三种多糖对蛋白质非酶糖基化反应的终端产物(AGEs)和醛糖还原酶(AR)形成的抑制作用。结果表明,在对AGEs形成的抑制过程中,第四周时OPMC II的抑制作用较强,且强于同剂量的氨基胍;在对AR活性的抑制作用中,OPMC II在三种多糖样品中抑制作用最强,但明显低于阳性对照物依帕司他。
Cactus was used as a raw material to extract polysaccharides, which were concentrated by ultrafiltration and fractionated with different concentrations of ethanol to obtain 55% ethanol precipitated polysaccharide (OPMC I), further ethanol concentration increased to 80% precipitated polysaccharide (OPMCII), and disposable 80 % ethanol precipitated polysaccharide (OPMCIII). The inhibitory effects of three polysaccharides on the formation of non-enzymatic glycation end products (AGEs) and aldose reductase (AR) were compared. The results showed that in the inhibition of the formation of AGEs, OPMC II had a stronger inhibitory effect at the fourth week and was stronger than the same dose of aminoguanidine. In the inhibition of AR activity, OPMC II was found in three polysaccharide samples. The most potent inhibition was but significantly lower than the positive control Epalrestat.