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目的研究血管活性肠肽(VIP)对裸鼠移植瘤CD80、CD86表达的影响。方法建立裸鼠皮下实体瘤模型,当接种了MKN45实体瘤的裸鼠的肿瘤体积约为30 mm3时随机分为VIP组、VIP受体拮抗剂组、对照组3组,每组6只。皮下注射VIP或VIP受体拮抗剂10μg.100 ml-1.d-1,对照组PBS 100μl/d。4周后处死裸鼠,取出移植瘤,用免疫组织化学方法检测移植瘤组织中CD80、CD86蛋白表达的变化。结果 CD80、CD86在VIP处理组、VIP受体拮抗剂处理组、对照组瘤块组织中均有表达。VIP组CD80的表达明显低于VIP受体拮抗剂组(P<0.05);VIP组CD86蛋白的表达显明低于对照组(P<0.05);而其他各组间两者的表达差异均无统计学意义(P>0.05)。结论血管活性肠肽抑制裸鼠移植瘤中CD80、CD86的表达。
Objective To study the effect of vasoactive intestinal peptide (VIP) on the expression of CD80 and CD86 in nude mice xenografts. Methods The model of subcutaneous solid tumor in nude mice was established. When the tumor volume of nude mice inoculated with MKN45 solid tumor was about 30 mm3, they were randomly divided into VIP group, VIP receptor antagonist group and control group with 6 mice in each group. Subcutaneous injection of VIP or VIP receptor antagonist 10μg.100ml-1.d-1, the control group PBS 100μl / d. After 4 weeks, the nude mice were sacrificed and the xenografts were removed. The expression of CD80 and CD86 in tumor tissue was detected by immunohistochemistry. Results CD80 and CD86 were expressed in VIP treated group, VIP receptor antagonist treated group and control group. The expression of CD80 in VIP group was significantly lower than that in VIP receptor antagonist group (P <0.05). The expression of CD86 protein in VIP group was significantly lower than that in control group (P <0.05), but there was no statistical difference between the other groups Significance (P> 0.05). Conclusion Vasoactive intestinal peptide can inhibit the expression of CD80 and CD86 in xenografts in nude mice.