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目的:研究二氢欧山芹醇当归酸酯在家兔体内的药动学。方法:乙腈沉淀蛋白后以高效液相色谱法进行测定。色谱条件为Agilent ZORBAX Eclipse XDB C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-水(70∶30),流速1mL.min-1,检测波长320nm。采用DAS2.0程序计算药动学参数。结果:二氢欧山芹醇当归酸酯在0.25～96.0mg.L-1线性关系良好(R2=0.999 2),最低检测限为0.25mg.L-1。高、中、低浓度的日内和日间变异系数均小于10%,平均回收率>90%。二氢欧山芹醇当归酸酯ig药动学符合二室模型,主要药动学参数为t1/2α=(0.162±0.021)h,t1/2β=(4.9±0.4)h,Cmax=(75.5±1.6)mg.L-1,Vd=(0.127±0.011)L.kg-1,CL=(0.51±0.04)L.h-1.kg-1,AUC0-t=(35.7±1.4)mg.L-1.h-1。结论:二氢欧山芹醇当归酸酯在体内分布迅速,消除较慢。
OBJECTIVE: To study the pharmacokinetics of dihydro-docetaxel angelica in rabbits. Methods: After the protein was precipitated by acetonitrile, it was determined by high performance liquid chromatography. The chromatographic conditions were Agilent ZORBAX Eclipse XDB C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase of methanol-water (70:30) at a flow rate of 1 mL.min-1 and a detection wavelength of 320 nm. Pharmacokinetic parameters were calculated using the DAS 2.0 program. Results: There was a good linearity between 0.25 and 96.0 mg.L-1 (R2 = 0.999 2) and the lowest detection limit was 0.25 mg.L-1. High, medium and low concentrations of intra-day and inter-day coefficient of variation were less than 10%, the average recovery rate> 90%. The pharmacokinetics of Dihydropiper carnitine angelica ig fitted the two compartment model. The main pharmacokinetic parameters were t1 / 2α = (0.162 ± 0.021) h, t1 / 2β = (4.9 ± 0.4) h, Cmax = (75.5 ± 1.6 ) mg.L-1, Vd = (0.127 ± 0.011) L.kg-1, CL = (0.51 ± 0.04) Lh-1.kg-1 and AUC0-t = (35.7 ± 1.4) mg.L-1. h-1. Conclusion: Dihydropalmatine angelica is rapidly distributed in the body and eliminated slowly.