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目的探讨人脑星形细胞瘤发生发展中相关基因及分类特征基因的表达。方法用含13939种人基因的BioStarH140S芯片,以正常脑及18例胶质瘤组织总RNA制备的探针杂交芯片,ScanArray4000扫描信号,提取脑及不同级别星形细胞瘤的差异基因并行生物信息分析,Hierarchical聚类提取差异基因的特征。结果星形细胞瘤中筛选出438条(3.14%)差异表达基因;信息分析与细胞信号、细胞骨架和运动、癌基因及抑癌基因等多类基因密切相关;与分类相关的特征基因有MAP7、DBCCR1、PCDHA5、KCNAB1、NAP1L2等。表达谱将星形细胞瘤分成两类,与临床组织病理分类基本一致。结论芯片是基因分析和筛选肿瘤标志性基因的有效手段,可客观分析星形细胞瘤发展及预后;分类特征基因为星形细胞瘤侵袭性和预后判断提供依据,有助于临床诊治。
Objective To investigate the expression of related genes and taxonomic genes in the development of human astrocytomas. METHODS: BioStarH140S chip containing 13,939 human genes was used to probe the differential expression of differentially expressed genes in brain and different grades of astrocytomas by scanning probe hybridization chip with ScanArray4000 in normal brain and 18 glioma tissues. , Hierarchical clustering to extract the characteristics of different genes. Results 438 (3.14%) differentially expressed genes were found in astrocytomas. The information analysis was closely related to many genes such as cell signal, cytoskeleton and movement, oncogenes and tumor suppressor genes. The related genes were classified as MAP7 , DBCCR1, PCDHA5, KCNAB1, NAP1L2 and the like. The expression profile divided astrocytomas into two categories, which were basically the same as the clinical histopathological classification. Conclusion The chip is an effective method for gene analysis and screening of tumor marker genes, which can objectively analyze the development and prognosis of astrocytoma. The taxonomic characteristics of genes provide the basis for invasiveness and prognosis of astrocytomas, which is helpful for clinical diagnosis and treatment.