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用抗ＦＯＳ和抗酪氨酸羟化酶双重免疫组织化学方法对大鼠孤束核内儿茶酚胺能神经元在胃伤害性刺激后的ｃｆｏｓ表达进行了观察．光镜下见到孤束核内出现３种不同的阳性细胞，即ＦＯＳ、酪氨酸羟化酶单标细胞、ＦＯＳ／酪氨酸羟化酶双标细胞．这３种标记细胞主要分布在延髓的中、尾段孤束核内侧亚核、连合亚核以及孤束核的背侧周边区．其中，ＦＯＳ单标细胞数量最多，其次是ＦＯＳ／酪氨酸羟化酶双标细胞，酪氨酸羟化酶单标细胞数量最少．结果表明，胃受到伤害性刺激后，其非特异性一般内脏信息主要传入到延髓孤束核的３个亚区．发现大多数酪氨酸羟化酶标记细胞中同时显示出ＦＯＳ阳性产物．
The anti-FOS and anti-tyrosine hydroxylase double immunohistochemistry on the rat solitary tract nucleus catecholaminergic neurons in gastric noxious stimulation c fos expression was observed. Light microscope to see the nucleus of the solitary tract there are three different kinds of positive cells, namely, FOS, tyrosine hydroxylase single-labeled cells, FOS / tyrosine hydroxylase double-labeled cells. These three kinds of labeled cells mainly located in medial and caudal segments of medulla oblongata, subnuclei medial to nucleus tractus solitarius, conjunctiva subunit and dorsal marginal zone of nucleus tractus solitarius. Among them, the number of FOS single labeled cells was the highest, followed by the double labeled cells of FOS / tyrosine hydroxylase and the lowest number of single labeled cells of tyrosine hydroxylase. The results showed that after the stomach was noxiously stimulated, its nonspecific general visceral information was mainly transmitted to the three subregions of the medulla oblongata nucleus. Most tyrosine hydroxylase labeled cells were found to show both FOS positive products.