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Objective:To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr,fruit inα-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents,α-amylase inhibitory activity,and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.Results:Presence of saponin,steroid and terpenoid were identified in the extract.The results showed that fruit extract had moderateα-amylase inhibitory activity[IC_(50)value=(3.638±0.190)mg/mL]as compared to acarbose.Moreover at 500 mg/kg and 1000 mg/kg doses fruit extract significantly(P<0.05 and P<0.01 respectively)reduced fasting blood glucose level in normal rats as compared to glibenclamide(5 mg/kg).In oral glucose tolerance test,500 mg/kg dose significantly reduced blood glucose level(P<0.05)at 2 h but 1000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h(P<0.05 and P<0.01 respectively)whereas glibenclamide(5 mg/kg)significantly reduced glucose level at every hour after administration.Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test.Conclusion:These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.
Objective: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr, fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo). Methods: Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats. Results: Presence of saponin, steroid and terpenoid were identified in the extract. that fruit extract had moderate α-amylase inhibitory activity [IC 50 value = (3.638 ± 0.190) mg / mL] as compared to acarbose.Moreover at 500 mg / kg and 1000 mg / kg doses fruit extract significantly (P <0.05 and P <0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg / kg) .In oral glucose tolerance test, 500 mg / kg dose significantly reduced blood glucose level (P <0 Glutamine (5 mg / kg) significantly reduced glucose level at every hour after administration (P <0.05 and P <0.01 respectively) . Overall time effect is also considered extremely significant with F value = 23.83 and P value = 0.0001 in oral glucose tolerance test. Confc: These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.