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成的蛋白晶体对蚊虫和蚋的幼虫有杀灭作用,而且在溶解后对大多数细胞有溶胞作用。此种晶体系由分子量为130K、65K和28K的多肽组成。本文报道用单克隆抗体亲和层析法去除28K肽后,观察晶体其余成分对蚊的作用。采用苏云金杆菌以色列亚种LB一株的自发抗氯霉素突变体922 910作为晶体来源,配制工一2mg/ml的晶体蛋白水悬液,用1M NaOH调节pH至n.7,在室温搅拌5小时,再用pH7。0 fris-HCl缓冲液调节至pH8.o,经10 000g离心后取上清液备用。用
Crystal protein into the mosquito and gall bladder larvae have a killing effect, but also in the majority of cells after lysis. Such a crystal system consists of polypeptides with molecular weights of 130K, 65K and 28K. This article reports the removal of 28K peptide by monoclonal antibody affinity chromatography to observe the effect of the rest of the crystal on mosquitoes. A spontaneous chloramphenicol resistance mutant strain 922 910 from Bacillus thuringiensis subtilisin LB strain was used as a source of crystals to prepare a crystal protein suspension of 2 mg / ml. The pH was adjusted to n.7 with 1 M NaOH and stirred at room temperature for 5 H, then adjust pH7.0 fris-HCl buffer to pH8.o, centrifuged at 10 000g after taking the supernatant spare. use