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1988～1995年对福建西番莲病毒病的发生为害进行调查,发现在西番莲栽培区病毒病普遍发生,发病率通常在30％～40％,严重的达90％以上。田间症状主要表现为叶片环斑、皱缩、花叶、环斑花叶,死顶和果实木质化等。从田间病株采集40份样本,经电镜观察和采用A蛋白夹心ELISA(PAS-ELISA),用西番莲木质化病毒(PWV)、西番莲黄花叶病毒(PFYMV)、紫果西番莲花叶病毒(GMV)、烟草花叶病毒(TMV)及黄瓜花叶病毒(CMV)的抗血清进行测定,其中36个样本检测出CMV,表明福建西番莲病毒病的主要病原为CMV。与此同时,对西番莲上CMV进行亚组鉴定,采用鉴别寄主和单、多克隆抗体双夹心ELISA(DAS-ELISA)对36个CMV阳性样本进行测定,结果35个属CMV亚组Ⅰ,1个属CMV亚组Ⅱ,表明田间以CMV亚组Ⅰ分离物占绝对优势。
From 1988 to 1995, the incidence of passiflora virus disease in Fujian Province was investigated. It was found that the incidence of virus disease was generally 30% -40% and severe in over 90% in the cultivation area of passion flower. Symptoms of the main symptoms of the leaves ring spot, shrinkage, mosaic, ring macular mosaic, dead wood and fruit and so on. A total of 40 samples were collected from the field strain and observed by electron microscopy and protein A sandwich ELISA (PAS-ELISA), using PWV, PFYMV, (GMV), Tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) antisera. Among them, CMV was detected in 36 samples, indicating that the main pathogen of Fujian Passiflora virus disease was CMV. At the same time, 36 CMV positive samples were determined by subcutaneous sandwich ELISA (DAS-ELISA) with differential host and single and polyclonal antibody. The results showed that 35 CMV subgroups Ⅰ, One belongs to the CMV subgroup II, indicating that the CMV subgroup I isolates dominate in the field.