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目的介绍大鼠清醒状态下尾静脉注射的快速操作技巧与体会,提高操作成功率。方法采用自制大鼠束缚衣结合操作者轻按的方法固定大鼠,一人辅助固定,一人穿刺,一人注药,在注射过程中尽量减少对鼠尾的损伤和对大鼠产生的应激,严格规定进针点和穿刺次数,限制一次性最大给药剂量和注射速度,并采取将进针与注射药液的部位分开等方法避免药物污的染和浪费。结果本法操作快速、可靠、稳定、重复性好、动物配合度高。结论本法避免了药物的浪费和污染,对于药物昂贵的尾静脉注射实验此做法尤为推荐。
Objective To introduce the rapid operation technique and experience of caudal vein injection in rat awake state and to improve the operation success rate. METHODS: The rats were restrained by using the self-made rat binding clothing combined with the operator’s light press method. One person assisted the fixation, one puncture and one injection, minimized the damage to the rat tail and the stress on the rats during the injection. Provisions of the needle point and the number of punctures, limit the maximum one-time dose and injection speed, and take the injection needle and the injection of liquid parts and other methods to avoid contamination and waste of drug contamination. Results This method is fast, reliable, stable, reproducible, animal with high. Conclusion This method avoids the waste and pollution of drugs. This method is especially recommended for expensive tail vein injection experiments.