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本文旨在观察血管内放射对冠状动脉球囊扩张术后细胞外信号调节激酶 1/ 2 (ERK1/ 2 )及c fos基因表达的影响。实验对猪的左冠状动脉前降支或回旋支行球囊行过度扩张术 ,术后即刻通过血管内放射治疗系统对猪冠状动脉损伤局部给予 2 0Gy的放射剂量 ,分别在术后 3d和 30d处死动物 ,留取目标血管组织。通过反转录 聚合酶链反应定量检测血管内放射对球囊扩张术后血管组织c fosmRNA的表达 ,采用生化方法测定血管组织ERK1/2的活性。结果表明血管内放射治疗明显抑制了球囊扩张术后 3dERK1/ 2活性和c fosmRNA的表达 (2 0 5 % ,P <0 0 1;47 7% ,P <0 0 5 ) ,而对球囊扩张术后 30d的ERK1/ 2活性和c fosmRNA表达无显著影响。这提示ERK1/ 2和c fos可能参与了血管内放射抑制球囊扩张术后早期再狭窄过程。
This article aims to observe the effects of intravascular radiation on the expression of extracellular signal-regulated kinase 1/2 (ERK1 / 2) and c fos after coronary artery balloon surgery. Experimental pigs left anterior descending coronary artery or branch balloon balloon overdispersion, immediately after intravascular radiation therapy system of pig coronary lesions locally given 20 Gy dose, were killed at 3d and 30d after surgery Animals, to retain the target vascular tissue. The expression of c fos mRNA in vascular tissue after balloon dilation was quantitatively determined by reverse transcription-polymerase chain reaction (RT-PCR). The biochemical method was used to determine the activity of ERK1 / 2 in vascular tissue. The results showed that intravascular radiation therapy significantly inhibited 3dERK1 / 2 activity and c fos mRNA expression after balloon dilatation (20%, P <0.01; 477%, P <0 05) No significant effect of ERK1 / 2 activity and c fos mRNA expression was observed 30 days after dilation. This suggests that ERK1 / 2 and c fos may be involved in intravascular radiation suppression of early restenosis after balloon dilatation.