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探讨端粒酶活性在肝癌演进中的变化,用3’-甲基-4-二甲基氨基偶氮苯(3’-Me-DAB)作诱变剂,建立SD大鼠肝癌变动物模型.自灌胃之日起,分别在2、4、6、8、10、12、14、17w分批处死大鼠,取其肝脏,进行病理分级,然后用TRAP-ELISA法检测肝癌变各时期端粒酶活性,结果显示:在轻度炎性反应期(LI)端粒酶活性高于对照组,呈极显著差异(P<0.01);重度炎性反应期(HI)端粒酶活性有所下降,但仍高于对照组,且呈显著性差异(P<0.05);至腺瘤样增生(NH)和肝内胆管细胞癌(CCC)时端粒酶活性再次升高,与对照组相比呈极显著差异(P<0.01).提示端粒酶活性增高可能是肝癌变进程中的重要事件,与肝癌的发生发展密切相关.
To investigate the changes of telomerase activity in the development of hepatocellular carcinoma (HCC), a rat model of hepatocarcinogenesis was established by using 3’-methyl-4-dimethylaminoazobenzene (3’-Me-DAB) as a mutagen. From the day of gavage, the rats were killed in batches on 2, 4, 6, 8, 10, 12, 14 and 17 weeks respectively, and the liver was taken for pathological grading. Then the TRAP- The results showed that telomerase activity in mild inflammatory reaction phase (LI) was significantly higher than that in control group (P <0.01), and telomerase activity in severe inflammatory reaction phase (HI) (P <0.05). The activity of telomerase in adenoma-like hyperplasia (NH) and intrahepatic cholangiocarcinoma (CCC) increased again. Compared with the control group (P <0.01), suggesting that the increase of telomerase activity may be an important event in the course of hepatocellular carcinogenesis, which is closely related to the occurrence and development of hepatocellular carcinoma.