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目的探讨细胞色素P450 1A1和2D6基冈多态性与慢性苯中毒易感性的关系。方法采用病例-对照研究,选择152名苯中毒工人为病例组,152名接触苯而无中毒表现的工人为对照组。采用限制性片段聚合酶链反应(PCR—RFLP)技术榆测CYP1A1基因3’端非编码区Msp I和CYP2D6第1外显子c．188位点、g．212位点多态性。结果携带CYP1A1 Msp I T／T基因型的个体发生苯中毒的危险性是携带有CYP1A1 Msp I T／C或C／C基因型的个体的1．32倍(95％CI:1．05～1．65, P=0．02);在不吸烟的人群中,携带CYP1A1 Msp I T/T基因型的个体发生苯中毒的风险性是携带T/C或C／C基因型的个体的1．56倍(95％CI:1．15～2．12,P=0．003);携带有CYP2D6 c．188 C／C或C／T基因型个体发生苯中毒的危险性足携带有CYP2D6 c．188 T/T基因型的个体的1．23倍(95％CI:1．05～1．42,P=0．01),在不吸烟的人群中,携带CYP2D6 c．188 C／C或C/T基因型个体发生苯中毒的危险性是携带有CYP2D6 c．188 T／T基因型的个体的1．23倍(95％CI:1．04～1．47,P=0．01)。未发现研究对象的CYP2D6g．212位点存在多态性。结论携带CYP1A1 Msp J T/T基因型、CYP2D6 c．188 C／C和C/T基因型个体对苯中毒可能易感。
Objective To investigate the relationship between the susceptibility of chronic benzene poisoning and the genetic polymorphisms of cytokines P450 1A1 and 2D6. Methods A case-control study was conducted in which 152 workers with benzene poisoning were selected as the case group and 152 workers exposed to benzene without toxicity were selected as the control group. The 3 ’untranslated region Msp I and CYP2D6 exon 1 of CYP1A1 gene were detected by PCR-RFLP. 188 sites, g. 212 locus polymorphism. Results The risk of benzene poisoning in individuals carrying the CYP1A1 Msp IT / T genotype was 1.32 times greater (95% CI: 1.05-1.65) than individuals with the CYP1A1 Msp IT / C or C / C genotype , P = 0.02). In non-smokers, individuals with CYP1A1 Msp IT / T genotype had 1.56 times more risk of benzene poisoning than those with T / C or C / C genotype 95% CI: 1.15-2.12, P = 0.003); carrying CYP2D6c. 188 C / C or C / T genotype The risk of benzene poisoning in individuals carrying CYP2D6 c. 1.23-fold (95% CI: 1.05-1.42, P = 0.01) in individuals with the 188 T / T genotype and CYP2D6c in non-smokers. 188 C / C or C / T genotype benzene poisoning risk of individuals carrying CYP2D6 c. 1.23-fold (95% CI: 1.04-1.47, P = 0.01) for the 188 T / T genotype. No research object CYP2D6g was found. 212 loci polymorphism. Conclusion Carrying CYP1A1 Msp J T / T genotype, CYP2D6 c. Individuals with 188 C / C and C / T genotypes may be susceptible to benzene poisoning.