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目的:观察重楼皂苷Ⅰ(polyphyllin I,PPⅠ)联合加热抗胃癌MKN-45细胞株的效应。方法:PPⅠ与MKN-45细胞共培养,采用MTT法检测PPⅠ对细胞增殖的影响,流式细胞仪检测细胞凋亡变化;PPⅠ联合加热处理MNK-45细胞,集落形成试验检测加热对PPⅠ抗MNK-45细胞抗瘤活性的影响,Western blot法检测p21蛋白表达变化。结果:不同浓度PPⅠ作用于MKN-45胃癌细胞株24h和48h后,细胞存活率显著降低,呈时间和剂量依赖关系。PPⅠ作用于MKN-45细胞24 h后,MNK-45细胞凋亡率由(4.3±1.1)%升高至(37.8±3.5)%。PPⅠ(IC30)联合加热后,细胞存活率由(61.2±3.4)%降至(40.7±3.1)%(P<0.05)。PPⅠ能上调MKN45细胞p21蛋白表达,这一效应能被加热增强。结论:PPⅠ对胃癌MKN-45细胞株有明显增殖抑制和诱导凋亡的作用,可能与其上调p21蛋白表达有关,联合加热能增强这一效应。
Objective: To observe the effect of polyphyllin I (PPI) combined with heating on gastric cancer MKN-45 cell line. METHODS: PPI was co-cultured with MKN-45 cells. MTT assay was used to detect the effect of PPI on cell proliferation. Flow cytometry was used to detect the apoptosis of PPAR-MNK-45 cells. Colony formation assay -45 cells, the changes of p21 protein expression were detected by Western blot. Results: The survival rate of MKN-45 gastric cancer cells treated with different concentrations of PPⅠ for 24 hours and 48 hours significantly decreased in a time-and dose-dependent manner. The apoptosis rate of MNK-45 cells increased from (4.3 ± 1.1)% to (37.8 ± 3.5)% after treated with PPⅠ for 24 h. The cell viability decreased from (61.2 ± 3.4)% to (40.7 ± 3.1)% after PPI (IC30) combined heating (P <0.05). PP Ⅰ can up-regulate p21 protein expression in MKN45 cells, and this effect can be enhanced by heat. Conclusion: The effect of PPⅠ on MKN-45 cell line can be obviously inhibited and induced by apoptosis, which may be related to its up-regulation of p21 protein expression. Combined heat can enhance this effect.