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【目的】利用单针注射等温微量量热滴定法(SIITC)检测尿素水解菌对Hg~(2+)的耐受性。【方法】与传统的细胞生长曲线法和基于尿素水解的p H变化曲线相对比,应用SIITC研究耐盐尿素水解菌Staphylococcus succinus对不同浓度Hg~(2+)的耐受性,通过相关性分析验证SIITC检测细菌对重金属胁迫耐受性的可行性。【结果】S.succinus菌吸附水解尿素的最大热量交换速率和累积热量值随Hg~(2+)浓度升高而降低。最大热量交换速率由不含Hg~(2+)的-45μJ/s降至10μmol/L Hg~(2+)处理的-20μJ/s,吸收热量由-1 291.90μJ下降到-535.75μJ;不同浓度Hg~(2+)胁迫下S.succinus菌吸附水解尿素的反应热量值与溶液p H以及OD_(600)的相关系数r分别达到0.928和0.955。【结论】SIITC可用来检测Hg~(2+)对尿素水解菌S.succinus的毒性。
【Objective】 The tolerance of Hg 2+ to urea-hydrolyzed bacteria was detected by single-needle isothermal microcalorimetry (SIITC). 【Method】 Compared with the traditional curve of cell growth and p H curve based on urea hydrolysis, SIITC was used to study the tolerance of salt-resistant urea-hydrolyzate Staphylococcus succinus to Hg 2+ at different concentrations. Through correlation analysis To verify the feasibility of SIITC to detect bacteria tolerance to heavy metal stress. 【Result】 The results showed that the maximum heat exchange rate and cumulative heat value of S. succinus adsorbed hydrolyzed urea decreased with the increase of Hg 2+ concentration. The maximum heat exchange rate decreased from -45μJ / s without Hg 2+ to -20μJ / s with 10μmol / L Hg 2+, and the absorbed heat decreased from -1 291.90μJ to -535.75μJ. Under the condition of Hg 2+ stress, the correlation coefficient between the reaction calorie value and the solution p H and OD 600 of S.succinus bacteria reached 0.928 and 0.955 respectively. 【Conclusion】 SIITC can be used to detect the toxicity of Hg 2+ to urea-soluble S.succinus.