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目的:建立测定神香草中迷迭香酸和总多糖含量的方法。方法:采用高效液相色谱法测定神香草中迷迭香酸的含量。色谱柱为Shim-pack VP-ODS,流动相为乙腈-0.5%甲酸(17∶83,V/V),流速为1.0 ml/min,检测波长为330 nm,柱温为30℃,进样量为10μl。采用紫外-可见分光光度法测定神香草中总多糖的含量,检测波长为620 nm。结果:迷迭香酸检测质量浓度线性范围为10.86~54.30μg/ml(r=0.999 8);精密度、稳定性、重复性的RSD<1.0%;加样回收率为98.20%~101.79%,RSD为1.68%(n=6)。总多糖检测质量浓度线性范围为1.996~15.97μg/ml(r=0.999 6);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为96.62%~101.68%,RSD为1.88%(n=6)。结论:该方法操作简便、重复性好,可用于神香草中迷迭香酸和总多糖含量的测定。
Objective: To establish a method for the determination of rosmarinic acid and total polysaccharides in Shen vanilla. Methods: The contents of rosmarinic acid in Shen vanilla were determined by HPLC. The chromatographic column was Shim-pack VP-ODS with a mobile phase of acetonitrile-0.5% formic acid (17:83, V / V) at a flow rate of 1.0 ml / min with a detection wavelength of 330 nm and a column temperature of 30 ° C. 10 μl. The content of total polysaccharides in Herba Vannamei was determined by UV-Vis spectrophotometry. The detection wavelength was 620 nm. Results: The linear range of determination of rosmarinic acid was 10.86 ~ 54.30μg / ml (r = 0.999 8). The RSD of precision, stability and repeatability was less than 1.0%. The average recoveries were 98.20% ~ 101.79% RSD was 1.68% (n = 6). The linear range of total polysaccharide detection was 1.996-15.97μg / ml (r = 0.999 6). The RSD of precision, stability and reproducibility was less than 2.0%. The recoveries were 96.62% -101.68% with RSDs of 1.88 % (n = 6). Conclusion: The method is simple, reproducible and can be used for the determination of rosmarinic acid and total polysaccharide in Herba Vannamei.