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作者用单克隆抗体和免疫金方法,对正常人外周血B淋巴细胞的形态学和膜表现型进行研究,发现B淋巴细胞亚型分为:(Ⅰ)40％的细胞与OKIa/FMC 4有中等强度反应,其核/浆比率相对高,染色质显著凝聚,有短的内质网(ER)。半数这种细胞有明显的高尔基氏区和与其联合的囊泡,大多数无核仁,仅稍有绒毛样外形;(Ⅱ)40％的细胞表面具有强的HLA-Dr抗原,连续切片显示有1—2个核仁,核为圓形或卵圆形。其染色质与上型相比,凝集程度不一致,部分这类细胞类似β-PLL细胞,如:核常为环形,核/浆比率低,核周染色质显著凝聚,高尔基氏区明显,ER较长。这种细胞的胞浆中除了苍白颗粒外,还有中等密度溶酶体颗粒群;(Ⅲ)10％的B淋巴细胞有较多绒毛,形态类似HCL
The authors used monoclonal antibodies and immunogold methods to study the morphology and membrane phenotype of B lymphocytes in normal human peripheral blood and found that B lymphocyte subtypes are divided into: (1) 40% of cells and OKIa / FMC 4 have Moderate-intensity reactions with relatively high core / pulp ratios, significant chromatin condensation, and short ER. Half of these cells have distinct Golgi regions and associated vesicles, most of which have no nucleoli and only a slightly villous appearance; (II) 40% of the cells have a strong HLA-Dr antigen on the surface and serial sections show 1-2 nucleoli, nucleus round or oval. The chromatin and the type compared to the degree of agglutination inconsistent, some of these cells similar to the β-PLL cells, such as: nuclear often ring, nuclear / plasma ratio was low, significant condensation of perinuclear chromatin, Gorky area significantly, ER long. In addition to the pale particles, there are also medium density lysosome particles in the cytoplasm of this cell; (3) 10% of B lymphocytes have more villi and are similar in morphology to HCL