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为了观察营养不良对幼鼠海马齿状回 (DG)和脑室下层 (SVZ)的细胞增殖和神经发生的影响 ,采用 5 -溴 -2 -脱氧尿苷(Brd U)标记结合免疫组织化学方法对脑切片分别进行 Brd U、Tu J1(β tubulin,β微管蛋白 )及 GFAP(胶质纤维酸性蛋白 )反应或双重反应。结果表明 ,营养不良幼鼠齿状回的细胞增殖和神经生发明显高于营养良好的幼鼠而脑室下层的细胞增殖数量在两者却无明显差异。在齿状回 ,新生的细胞中大约有 5 0 %为新生的神经元 ,10～ 2 0 %为神经胶质细胞。本文结果提示 ,幼鼠海马齿状回的细胞增殖和神经生发可能因营养不良而增加 ,这些新生的细胞可能对日后某些海马依赖性行为产生一定的影响
In order to observe the effect of malnutrition on cell proliferation and neurogenesis in hippocampal dentate gyrus (DG) and subventricular zone (SVZ) of young rats, we used 5 - bromo - 2 - deoxyuridine (BrdU) labeling combined with immunohistochemistry Brain sections were subjected to BrdU, Tu J1 (β tubulin, β tubulin) and GFAP (glial fibrillary acidic protein) reactions or double reactions, respectively. The results showed that the malnutrition of rat dentate gyrus cell proliferation and neurite outgrowth was significantly higher than that of the well-nourished young rats and the number of cells in the subventricular layer did not show any significant difference between the two. In dentate gyrus, about 50% of newborn cells are neonate neurons and 10 to 20% are glial cells. Our results suggest that cell proliferation and neurodevelopment in hippocampal dentate gyrus may be increased due to malnutrition and that these newborn cells may have some impact on certain hippocampus-dependent behaviors in the future