论文部分内容阅读
大量证据表明，兴奋性氨基酸（ｅｘｃｉｔａｔｏｒｙａｍｉｎｏａｃｉｄｓ，ＥＡＡｓ）是脊椎动物中枢神经系统的兴奋性神经递质，并参与伤害性信息的一级传递。但对ＮＭＤＡ受体在不同伤害性信息传递中的作用却颇有争议。本实验采用免疫组化方法显示脊髓Ｆｏｓ样免疫活性（Ｆｏｓ—ｌｉｋｅｉｍｍｕｎｏｒｅａｃｔｉｖｉｔｙ，ＦＬＩ）神经元，观察化学性伤害性刺激物福尔马林（５％，１５０μｌ）足底注射对脊髓ｃ—Ｆｏｓ表达的诱导，以及ＭＫ—８０１对福尔马林诱导脊髓Ｆｏｓ蛋白产生的影响。结果：福尔马林足底注射引起的Ｆｏｓ表达主要分布于脊髓背角的Ⅰ—Ⅱ层和Ⅴ－Ⅵ层；ＭＫ—８０１（０．６ｍｇ／ｋｇ，ｉ．ｐ．）几乎完全阻断福尔马林引起的脊髓Ｆｏｓ表达。这一结果表明，福尔马林刺激所引起的脊髓Ｆｏｓ蛋白表达，绝大多数由ＮＭＤＡ受体介导，提示ＮＭＤＡ受体在福尔马林化学性伤害性信息传递中，具有至关重要的作用。
There is ample evidence that excitatory amino acids (EAAs) are excitatory neurotransmitters in the vertebrate central nervous system and are involved in the primary transmission of nociceptive information. However, the role of NMDA receptors in different nociceptive transmission is controversial. In this study, Fos-likeimmunoreactivity (FLI) neurons in the spinal cord were detected by immunohistochemistry. The effect of formalin (5%, 150μl) chemical injection on the expression of c-Fos And the effect of MK-801 on formalin-induced Fos protein production in the spinal cord. RESULTS: The Fos expression in formalin-injected rats was mainly distributed in the layers Ⅰ-Ⅱ and Ⅴ-Ⅵ of the dorsal horn of the spinal cord. MK-801 (0.6mg / kg, i.p.) almost completely blocked the expression of Fos Marrow-induced spinal Fos expression. This result indicates that most of the Fos protein expression in the spinal cord induced by formalin stimulation is mediated by NMDA receptors, suggesting that NMDA receptors are of crucial importance in chemical-nociceptive information transmission in formalin effect.