通过RT-PCR技术检测大鼠颈椎脱臼法和机械性窒息处死后心肌GAPDH mRNA在两种死亡原因下相应时间点的含量变化,结果表明GAPDHmRNA含量均随死亡时间延长而逐渐降低(P<0.05),且与死亡时间呈线性关系,但是颈椎脱臼处死大鼠心肌组织的扩增产物含量要略高于窒息处死组含量,可为PMI推断提供一种新的观测指标。 The contents of GAPDH mRNA in rat myocardium at the corresponding time points after the death of cervical dislocation and mechanical asphyxia were detected by RT-PCR. The results showed that the content of GAPDH mRNA decreased with the prolongation of death (P <0.05) , But there was a linear relationship with the time of death. However, the content of amplification product in myocardial tissue of rats killed by cervical dislocation was slightly higher than that of asphyxial death group, which could provide a new observation index for PMI inference.