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目的:利用P815肿瘤动物模型观察IL-12对肿瘤动物模型抗肿瘤作用的效应。方法:将小鼠肥大细胞瘤P815特异抗原基因P1A克隆到真核表达质粒pCI-neo中;用P815细胞对DBA/2小鼠右腹侧皮下注射,构建P815小鼠肿瘤模型;以重组基因疫苗单独或与鼠IL-12真核表达质粒一起肌肉注射,观察肿瘤的消长、特异细胞毒T淋巴细胞激活和抗体的生成情况。结果:重组基因疫苗在体外有很好的表达,注射后细胞毒性T淋巴细胞(CTL)的杀伤效率为40%,IL-12共注射的CTL杀伤效率达到60%。免疫后,30%小鼠的肿瘤出现消退;同IL-12共注射则有50%的小鼠的肿瘤出现消退。两种情况下都不能检测到任何特异抗体的产生。结论:长期存在的IL-12可以持续刺激机体细胞免疫,使肿瘤的生存环境持续恶化,从而达到治疗肿瘤的目的。
OBJECTIVE: To observe the effect of IL-12 on the anti-tumor effect of tumor animal model by P815 tumor animal model. Methods: The mouse mastocytoma P815 specific antigen gene P1A was cloned into the eukaryotic expression plasmid pCI-neo. The P815 mouse tumor model was constructed by subcutaneous injection of P815 cells into the right ventral of DBA / 2 mice. Mice were injected intramuscularly with murine IL-12 eukaryotic expression plasmid to observe tumor growth, specific cytotoxic T lymphocyte activation and antibody production. Results: The recombinant DNA vaccine had good expression in vitro. The killing rate of cytotoxic T lymphocytes (CTL) after injection was 40% and the CTL killing efficiency of IL-12 co-injection was 60%. After immunization, tumors in 30% of mice subsided; in co-injection with IL-12, tumors in 50% of mice subsided. No specific antibody production could be detected in either case. CONCLUSION: Long-term existence of IL-12 can continuously stimulate the cellular immunity of the body and make the living environment of the tumor continue to deteriorate, so as to achieve the purpose of treating the tumor.