论文部分内容阅读
目的:探讨双氢睾酮（DHT）对人外周血早期内皮祖细胞（PB-EPCs）增殖、迁移功能的影响及RhoA/ROCK信号通路在其中的作用。方法:取健康成人外周血分离、培养出早期EPCs并鉴定。分别以不同浓度DHT(1、10、100 nmol/L)干预，得出最佳浓度和最佳作用时间后用于后续干预实验。分组：对照组、DHT、RhoA抑制剂C3 exoenzyme+DHT组、ROCK抑制剂Y-27632+DHT组。检测各组EPCs的增殖、迁移能力。ELISA法检测各组细胞上清液液中VEGF蛋白含量。结果:DHT在一定范围内呈浓度-时间依赖性促进EPCs增值、迁移功能，分别在浓度为10 nmol/L和24 h达到最大作用效果。与单纯10 nmol/L的DHT刺激组相比，C3 exoenzyme[(0.22±0.02) n vs (0.26±0.05),n P>0.05]和Y-27632[(0.21±0.04) n vs (0.26±0.05), n P>0.05]能够减弱DHT诱导的EPCs增殖，但差异无统计学意义。DHT对EPCs迁移能力的促进作用可被C3 exoenzyme[(35.26±4.27) n vs (46.92±5.46), n P0.05] can attenuate the proliferative capacities of EPCs induced by DHT compared with the DHT group, but there was no statistical significance. The influence of DHT on EPCs migrative capacities can be abolished by C3 exoenzyme [(35.26±4.27) n vs (46.92±5.46), n P<0.05] and Y-27632 [(33.61±5.33) n vs (46.92±5.46), n P<0.01]. C3 exoenzyme [(116.75±7.42) n vs (156.80± 21.74), n P<0.05] and Y-27632 [(121.73±5.33) n vs (156.80 ±21.74), n P<0.01] could noticeably attenuate DHT-induced EPCs secretion of VEGF respectively.n Conclusions:DHT can modulate EPCs proliferation, migration and the RhoA/ROCK pathway plays an important role in this process.