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作者采用组织化学方法,研究了人参皂甙在人参根中的分布部位。实验方法为:(1)取新鲜人参根的横切面压在硅胶 GF_(254)薄层板上,使成分转移至薄层上,用2％(W/V)硅钨酸-乙醇试剂喷雾,加热后即显紫色;(2)将新鲜根皮层分泌道中溢出的粘液状渗出物,用甲醇溶解,于薄层板上点样,氯伪-甲醇-水(6∶4∶1)展开,以皂甙检出剂1％硫酸铈的10％硫酸试液喷雾,加热显色,用 CS-910型双波长薄层扫描仪,在560nm 波长处,用反射法作线形扫描,并与人参皂甙标准品对照;(3)将自然干燥的人参根,制成2～3mm 厚的横切片,以
The authors used histochemical methods to study the distribution of ginsenoside in ginseng roots. The experimental methods were as follows: (1) The cross section of fresh ginseng root was pressed on silica GF_ (254) plate and the ingredients were transferred to thin layer and sprayed with 2% (w / v) silicotungstic acid- (2) The mucilaginous exudate in the fresh root cortex exudates was dissolved in methanol, spotted on a thin plate, and chloramphenicol-methanol-water (6: 4: 1) To saponin detection agent 1% cerium sulfate 10% sulfuric acid test solution spray, heat color, with CS-910-type dual-wavelength thin layer scanner at 560nm wavelength, with a linear scan method for reflection and ginsenosides standard Product control; (3) natural dried ginseng root, made of 2 ~ 3mm thick cross-section to