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利用离体肝脏灌流技术测定了 CL(二个实验组 ,CL给药量分别为 1× 1 0 - 8mol和 1× 1 0 - 6mol)对大鼠肝脏物质代谢及相关酶活性的影响 .结果表明 CL可使大鼠离体肝脏灌流液中尿素氮浓度下降 ,并有一定的剂量效应和时间效应 .在给药后灌流的 1、2、3、4 h内 ,与对照组相比 ,1× 1 0 - 6mol的 CL使大鼠离体灌流的肝脏产生的尿素氮分别下降了1 4 .79% ( P>0 .0 5 ) ,1 7.88 ( P>0 .0 5 ) ,2 6 .79 ( P<0 .0 5 )和 2 9.95 ( P<0 .0 1 ) . 1× 1 0 - 8mol的 CL具有类似的效应 .而 CL对大鼠离体肝脏灌流液中葡萄糖的水平影响不大 .CL可抑制大鼠肝组织中 GPT的活性 ,灌流 4 h后 1× 1 0 - 6mol的 CL使得肝组织中 GPT活性下降了 2 4 .6 5 % ( P<0 .0 5 ) ,但 1× 1 0 - 8mol的 CL仅使 GPT活性下降 7.5 0 % ( P>0 .0 5 ) .CL 还抑制大鼠肝组织中 G6 PDH的活性 ,1× 1 0 - 6mol的 CL可使肝脏中 G6 PDH活性下降 36 .0 4 % ( P<0 .0 5 ) ,同样1× 1 0 - 8mol的 CL对 G6 PDH活性影响不显著 .提示 CL可直接通过影响肝脏的氮代谢及相关酶的活性继而影响机体的物质代谢 .
The effects of CL (two experimental groups, CL dose of 1 × 10 ~ 8mol and 1 × 10 ~ 6mol, respectively) on the hepatic metabolism and related enzyme activities in rats were measured by ex vivo hepatic perfusion technique CL could decrease the concentration of urea nitrogen in isolated rat liver perfusion fluid and have certain dose effect and time effect.Compared with the control group, 1 × The level of urea nitrogen produced by isolated rat perfused liver was decreased by 14.79% (P> 0.05), 1 7.88% (P> 0.05), 26% respectively. 79 (P <0. 05) and 2 9.95 (P <0 .01) .1 × 10 -8 mol CL had a similar effect, whereas CL had no effect on the level of glucose in isolated rat liver perfusate CL can inhibit the activity of GPT in rat liver tissue, 1 × 10-6 mol CL after 4 h of perfusion can reduce the activity of GPT in liver tissue by 24.56% (P <0.05) 1 × 10 ~ 8 mol CL decreased the activity of GPT only by 7.5% (P> 0.05) .CL also inhibited the activity of G6 PDH in rat liver tissue, and the CL of 1 × 10 ~ 6 mol The hepatic G6 PDH activity decreased by 36.0% (P <0.05), and the same 1 × 10-8 mol CL had no significant effect on the activity of G6 PDH, suggesting that CL can directly affect the metabolism of the body through affecting the hepatic nitrogen metabolism and related enzyme activity.