论文部分内容阅读
目的了解东北林区啮齿动物和蜱类查菲埃立克体感染情况。方法采用巢式PCR和间接免疫荧光方法检测长白山林区野鼠和蜱中查菲埃立克体DNA及野鼠查菲埃立克体抗体。对DNA阳性扩增产物测序,进行同源性分析。结果 PCR检测各种蜱共97组,其中13组阳性,阳性率13.40%。不同蜱种中,全沟硬蜱、森林革蜱查菲埃立克体阳性率分别为20.83%和10.0%,嗜群血蜱(14组)和长角血蜱(5组)均为阴性;检测野鼠共132只,阳性19只,阳性率14.39%。其中,集安与宽甸野鼠查菲埃立克体阳性率分别为7.58%和21.21%,差异有统计学意义(χ2=3.9348,P>0.05)。荧光抗体试验检测鼠血清75份,E.c抗体阳性10份,阳性率13.33%。对鼠和蜱标本阳性扩增产物测序并作同源性比较,蜱株、鼠株与查菲埃立克体美国株、韩国株及我国云南株、新疆株核苷酸序列同源性为99.69%~100.00%,聚类分析显示检测株与查菲埃立克体参考株同处一个分支,与日本株及埃立克体属其它成员差异较大。结论长白山林区野鼠和蜱类查菲埃立克体感染普遍,全沟硬蜱和森林革蜱为主要媒介,野鼠为重要宿主动物。表明该地区存在单核细胞埃立克体病的自然疫源地。
Objective To understand the infection status of rodents and ticks in the forest area of Northeast China. Methods The nested PCR and indirect immunofluorescence method were used to detect Chafira Ehrlichiae and T. muridarum antibodies in wild and ticks of Changbai Mountain. DNA positive amplification products were sequenced for homology analysis. Results A total of 97 ticks were detected by PCR, of which 13 were positive and the positive rate was 13.40%. In different species of ticks, the positive rates of Chafizia crataii and Chaumes elliottii were 20.83% and 10.0%, respectively, and those of Phasianus population (14 groups) and C. longicornis (5 groups) were all negative. A total of 132 wild rats were detected, 19 were positive, the positive rate was 14.39%. Among them, the positive rates of Ji’an and Kuandian wild rats were 7.58% and 21.21%, respectively, with significant difference (χ2 = 3.9348, P> 0.05). Fluorescent antibody test 75 serum samples were detected, E.c antibody positive 10, the positive rate of 13.33%. The positive amplification products of murine and ticks were sequenced and compared for homology. The homology between ticks, rat strains and American Chafizenia fulgens strains, Korean strains, Yunnan strains and Xinjiang strains was 99.69 % ~ 100.00%. Cluster analysis showed that the test strain was located in the same branch with the reference strain of Chafiri Ehrlichiae, which was quite different from other strains of Japanese strain and Ehrlichia. Conclusion Infectious euphorbia inhabitation and ticks in Changbai Mountain are common, and Ixodes nigricans and forest ticks are the main vectors. Vole is an important host animal. Suggesting that there is a natural foci of mononuclear Ehrlichiosis in the area.