目的　探讨人类粒巨噬细胞集落刺激因子（ＧＭＣＳＦ） 受体（ＧＭＲ） 在ＮＩＨ３Ｔ３ 细胞中表达的功能特性。方法　将编码人ＧＭＲα和β亚单位的ｃＤＮＡ 转染到无人ＧＭＲ表达的小鼠ＮＩＨ３Ｔ３ 细胞中，并检测其阳性转染子在配体刺激后的增殖信号传导与酪氨酸磷酸化。结果　重建的功能性ＧＭＲα／β可以介导细胞增殖与细胞集落形成，并诱导βｃ、Ｊａｋ２、Ｓｈｃ 及Ｓｈｃ 相关蛋白Ｐ１４５（ＳＨＩＰ） 酪氨酸磷酸化，然而，人ＧＭＣＳＦ并不能激活仅含ＧＭＲα的ＮＩＨ３Ｔ３ 细胞出现有丝分裂信号表达。结论　人ＧＭＲα与β亚单位同时转染入ＮＩＨ３Ｔ３ 细胞后，可通过βｃ 磷酸化，激活Ｊａｋ２ 、Ｓｈｃ 和ＳＨＩＰ信号传导途径，而导致配体依赖性细胞生长与集落形成 Objective To investigate the functional characteristics of human GM-CSF receptor (GMR) expressed in NIH3T3 cells. Methods The cDNA encoding human GMRα and β subunits was transfected into mouse NIH3T3 cells without human GMR and the proliferation signal transduction of the positive transfectants after ligand stimulation was tested. The Results The reconstructed functional GMRα / β could mediate cell proliferation and colony formation, and induced tyrosine phosphorylation of βc, Jak2, Shc and Shc-associated protein P145 (SHIP). However, human GMCSF did not activate NIH3T3 cells containing only GM  Rα appear mitotic signal expression. Conclusions Simultaneous transfection of human GMRα and β subunits into NIH3T3 cells activates Jak2, Shc and SHIP signaling pathways through βc phosphorylation, leading to ligand-dependent cell growth and colony formation