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本试验采用水提取法、乙醇提取法对苦杏仁苷进行提取,并对两种提取方法进行比较。结果表明:水提取法比乙醇提取法提取率高,但乙醇提取法在纯化过程中更方便。水提取法的最佳工艺参数为:料液比1∶10的沸水直接提取,提取时间40min。乙醇提取法的最佳工艺参数为∶乙醇浓度60%、料液比1∶6、提取温度85℃、提取时间50min。用高效液相色谱法对水提取物和乙醇提取物进行了检测,测定了两种提取物中存在苦杏仁苷。
In this experiment, water extraction method, ethanol extraction of amygdalin extraction, and the two extraction methods were compared. The results showed that water extraction method was better than ethanol extraction method, but ethanol extraction method was more convenient in the purification process. The optimum extraction process parameters: the ratio of solid to liquid 1:10 direct extraction of boiling water, extraction time 40min. The optimum technological parameters of ethanol extraction were: ethanol concentration 60%, solid-liquid ratio 1: 6, extraction temperature 85 ℃, extraction time 50min. Water extracts and ethanol extracts were tested by high performance liquid chromatography and amygdalin was determined for both extracts.