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目的研究翻白草油抗呼吸道合胞病毒(RSV)作用。方法选取翻白草油进行抗RSV研究,采用细胞病变反应(CPE)和噻唑蓝(MTT)比色法观测翻白草油对RSV作用。结果 MTT和CPE试验结果表明翻白草油在3种作用方式(除细胞保护作用方式)下能够增强宿主HeLa细胞存活率,且细胞存活率与翻白草油浓度呈正相关,翻白草油对宿主HeLa细胞无保护作用,不能阻断RSV病毒进入细胞。结论翻白草油有抗RSV作用。
Objective To study the anti-respiratory syncytial virus (RSV) effect of Psidium. Methods The anti-RSV study was carried out on Pseudostellaria Burgdorferi oil. The effect of Pseudostellariae Blizzard on RSV was observed by cytopathic effect (CPE) and thiazolyl blue (MTT) colorimetry. Results The results of MTT and CPE showed that the activity of Hela cells was enhanced under three different modes of action (except for the cytoprotection), and the survival rate of Hela cells was positively correlated with the concentration of Ophiopogon japonicus, Host HeLa cells have no protective effect and can not block RSV virus entry into the cell. Conclusion Bupleurum oil has anti-RSV effect.