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为探讨褪黑素 (MEL)镇痛作用的机制 ,本文采用免疫组化方法结合计算机图像处理技术 ,观察了注射MEL对大鼠下丘脑弓状核内神经细胞的 β 内啡肽免疫反应的影响。实验大鼠分给药组及对照组 ,分别腹腔注射MEL 110mg/kg或配药液 ,1h后灌注取脑、冰冻切片 ,进行免疫组化染色 ,计算机图像处理技术测定染色脑片积分光密度 (IOD)和平均光密度 (OD)。结果显示 ,给药组大鼠弓状核内 β 内啡肽免疫反应明显减弱 ,IOD及OD也均显著减少 (P <0 0 1;P <0 0 5 ) ,表明MEL可致弓状核内 β 内啡肽含量减少 ,系MEL促进 β 内啡肽释放所致 ,从而发挥镇痛效应。
In order to explore the mechanism of melatonin (MEL) analgesic effect, immunohistochemistry and computer image processing techniques were used to observe the effect of MEL injection on β-endorphin immunoreactivity in hypothalamic arcuate nucleus neurons in rats . The experimental rats were divided into the treatment group and the control group. The rats were injected intraperitoneally with MEL (110 mg / kg) and the drug solution for 1 hour. The brain and frozen sections were harvested for immunohistochemical staining and computer image processing techniques to determine the integral optical density IOD) and average optical density (OD). The results showed that the β-endorphin immunoreactivity in the arcuate nucleus of the treated rats was significantly weakened, and the IOD and OD were also significantly decreased (P <0.01; P <0.05), indicating that MEL can cause arcuate nucleus β endorphin content decreased, Department of MEL promote β-endorphin release, which play the analgesic effect.