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目的观察核桃楸皮乙酸乙酯提取物(HYT)对小鼠前胃癌细胞MFC及人正常胃黏膜上皮细胞GES-1增殖的影响。方法从核桃楸皮中提取HYT,以不同浓度作用于MFC和GES-1细胞,MTT法检测HYT对MFC及GES-1细胞增殖的影响;流式细胞术检测MFC细胞周期变化及细胞凋亡情况。结果与阴性对照组比较,HYT可明显抑制MFC细胞增殖,促进细胞凋亡,对GES-1细胞无明显杀伤作用;HYT使MFC细胞阻滞于G0-G1和G2-M期。结论HYT可通过影响胃癌细胞周期,促进细胞凋亡,抑制其增殖。
Objective To observe the effects of ethyl acetate extract (HYT) on the proliferation of MFC and human normal gastric mucosal epithelial cells GES-1 in mouse gastric cancer cells. Methods HYT was extracted from Juglans mandshurica skin and treated with different concentrations of MFC and GES-1 cells. MTT assay was used to detect the effect of HYT on the proliferation of MFC and GES-1 cells. Flow cytometry was used to detect the changes of cell cycle and apoptosis . Results Compared with the negative control group, HYT significantly inhibited the proliferation of MFC cells and promoted the apoptosis of GES-1 cells. HYT inhibited the proliferation of MFC cells in G0-G1 and G2-M phases. Conclusion HYT can affect the cell cycle of gastric cancer, promote apoptosis and inhibit its proliferation.