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目的 :从健康人外周血中诱导高纯度树突状细胞 (DC)。方法 :将外周血单个核细胞用贴壁法分离出单核细胞 ,经多细胞因子联合诱导出DC。结果 :培养 8～ 9d后即可获得大量高纯度的成熟DC ,经流式细胞仪检测 ,所得细胞中高表达共刺激分子和粘附分子 ,表现为成熟DC的特征。结论 :利用多细胞因子组合可从外周血诱导大量成熟DC ,为DC的临床应用提供依据。
Objective: To induce high purity dendritic cells (DCs) from peripheral blood of healthy individuals. Methods: Peripheral blood mononuclear cells were isolated by adherent mononuclear cells, the combination of multiple cytokines induced DC. Results: A large number of mature DCs with high purity could be obtained after 8 ~ 9 days culture. By flow cytometry, costimulatory molecules and adhesion molecules were highly expressed in the cells, showing the characteristics of mature DC. Conclusion: The use of multiple cytokines combination can induce a large number of mature DCs from peripheral blood, providing the basis for the clinical application of DC.