液泡是植物细胞中特有的大型细胞器，具有重要的生理功能。本文报导了用双酶直接酶解法从烟草叶肉细胞中分离原生质体和完整液泡。在最适保存条件下，原生质体和液泡分别在３６和１２小时后，尚有一半保持活力。液泡内含有大量游离氨基酸，液泡膜ＡＴＰａｓｅ的最适ｐＨ为７．０，受Ｃｌ－激活，受ＮＯ３－抑制。 Bubble is a large organelle unique to plant cells and has important physiological functions. This paper reports the isolation of protoplasts and intact vacuoles from tobacco mesophyll cells by enzymatic digestion with two enzymes. Protoplasts and vacuoles remained viable after 36 and 12 hours, respectively, under optimum storage conditions. Vacuolar contains a large number of free amino acids, tonoplast ATPase optimum pH 7.0, by Cl - activation, by NO3 - inhibition.