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目的探讨高危型人乳头瘤病毒(HPV)及N-myc下游调节基因(NDRG1)在宫颈鳞癌发生、发展中的意义。方法收集2008年9月～2011年9月期间宫颈鳞癌及癌旁正常对照标本各82例,分别采用原位杂交法和免疫组织化学法检测标本中HPV16/18和NDRG1的表达情况。结果在宫颈鳞癌中HPV16/18和NDRG1的表达阳性率均明显高于癌旁正常组织(P均﹤0.05)。宫颈鳞癌组织中HPV16/18的表达与患者年龄、FIGO分期、肿瘤大小及淋巴结转移无关(P均﹥0.05);NDRG1的表达与FIGO分期、淋巴结转移密切相关(P均﹤0.05),而与患者年龄、肿瘤大小无关(P均﹥0.05)。结论 HPV16/18和NDRG1在宫颈鳞癌中的表达阳性率明显增高,证实高危HPV和NDRG1参与了宫颈鳞癌的发生、发展。
Objective To investigate the significance of high risk human papillomavirus (HPV) and N-myc downstream regulatory gene (NDRG1) in the occurrence and development of cervical squamous cell carcinoma. Methods Eighty-two cervical squamous cell carcinomas and adjacent normal tissues were collected from September 2008 to September 2011. The expression of HPV16 / 18 and NDRG1 in specimens were detected by in situ hybridization and immunohistochemistry respectively. Results The positive rates of HPV16 / 18 and NDRG1 in cervical squamous cell carcinoma were significantly higher than those in normal tissues (all P <0.05). The expression of HPV16 / 18 in cervical squamous cell carcinoma was not associated with age, FIGO stage, tumor size and lymph node metastasis (all P> 0.05). The expression of NDRG1 was closely related to FIGO stage and lymph node metastasis (all P <0.05) The age of patients and the size of tumor had no correlation (P> 0.05). Conclusion The positive rates of HPV16 / 18 and NDRG1 in cervical squamous cell carcinoma are significantly higher than those in normal cervical squamous cell carcinoma. It is confirmed that high-risk HPV and NDRG1 are involved in the occurrence and development of cervical squamous cell carcinoma.