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目的:研究抗凋零基因bcl-2和bax与卵巢上皮性肿瘤发生及预后的关系。方法:通过免疫组化方法,用抗bcl-2及抗bax抗体检测卵巢上皮肿瘤76份(良性27份、交界性24份、恶性25份)中bcl-2和bax的表达产物,并以正常卵巢12份做对照。结果:正常卵巢组织12份中bcl-2和bax阳性检出率为0,在良性、交界性及恶性肿瘤组织中bcl-2和bax的阳性检出率分别为7.4%(2/27)、16.7%(4/24)、36.0%(9/25),22.2%(6/27)、45.8%(11/24)、56.0%(14/25),良性与恶性肿瘤之间差异有显著性(P<0.05),而且bax的表达率比bcl-2高(P<0.05),bax在恶性肿瘤组Ⅰ级与Ⅲ级间差异有显著性(P<0.05)。结论:抗细胞凋零基因bcl-2和bax过度表达可能与卵巢上皮性肿瘤发生有关,推测bax不仅参与细胞凋零的调控,而且可能与肿瘤分化及预后有关。
OBJECTIVE: To study the relationship between anti-dying genes bcl-2 and bax and the occurrence and prognosis of epithelial ovarian tumor. Methods: The expression products of bcl-2 and bax in 76 epithelial ovarian tumors (27 benign, 24 borderline and 25 malignant) were detected by immunohistochemistry with anti-bcl-2 and anti-bax antibody. Ovary 12 copies of the control. Results: The positive rates of bcl-2 and bax in normal ovarian tissues were 0, and the positive rates of bcl-2 and bax were 7.4% (2/27) in benign, borderline and malignant tumors respectively. 16.7% (4/24), 36.0% (9/25), 22.2% (6/27), 45.8% (11/24) and 56.0% (14/25) respectively. The difference between benign and malignant tumors was significant (P <0.05), and the expression of bax was higher than that of bcl-2 (P <0.05). The difference of bax between grade Ⅰ and grade Ⅲ in malignant tumor group was significant (P <0.05). CONCLUSION: Overexpression of anti-apoptotic genes bcl-2 and bax may be related to ovarian epithelial tumor. It is speculated that bax is not only involved in the regulation of cell apoptosis, but also may be related to tumor differentiation and prognosis.