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及时发现脊髓灰质炎（脊灰）野病毒，是消灭脊灰工作中病毒学监测的首要任务。近期我国存在４个脊灰Ⅰ型野病毒基因型，其中Ｐ１／ＣＨＮ－ＪＸ８９和Ｐ１／ＣＨＮ－Ｒ９１为两个主要的流行基因型。在分析了我国大量脊灰野病毒ＶＰ１核酸序列的基础上，选取了１３３８ＪＸ８９病毒ＶＰ１５′端的９６个核苷酸片段（２４８０－２５７５），经ＰＣＲ扩增，克隆至ｐＵＣ／Ｔ７质粒，线性化后，用Ｔ７ＲＮＡ多聚酶制备ＲＮＡ探针，并将Ｄｉｇ标记物渗入探针分子中。经杂交试验，两个主要基因型病毒全部与此探针呈阳性反应，而其它基因型和疫苗相关病毒均为阴性反应，体现了较好的特异性与敏感性，而且实验结果由核酸序列分析等证实。脊灰野毒探针的应用在国际上尚未见报道，它克服了疫苗探针杂交中容易遗漏野毒株的缺点，直接查出野病毒。这对疫苗株中混有少量野毒株的分离物有重要意义。
Timely detection of poliomyelitis (poliovirus) wild-type virus is the number one priority in the virological monitoring of polio eradication efforts. Recently, there are 4 genotypes of wild-type Ⅰ wild-type poliovirus, of which P1 / CHN-JX89 and P1 / CHN-R91 are the two major genotypes. Based on the analysis of a large number of VP1 nucleic acid sequences of wild poliovirus in our country, a 96 nucleotides fragment (2480-2575) of VP15 ’of 1338JX89 virus was selected and cloned into pUC / T7 plasmid by PCR amplification. After linearization, RNA probes are prepared with T7 RNA polymerase and penetrate into the probe molecules. After hybridization test, all the two major genotype viruses were positive with this probe, while other genotypes and vaccine-associated viruses were negative reactions, showing good specificity and sensitivity, and the experimental results by nucleic acid sequence analysis Confirmed. Poliovirus probe application in the international community has not been reported, it overcomes the vaccine probe hybridization easy to miss the shortcomings of wild strains, direct detection of wild virus. This vaccine strains mixed with a small amount of wild strains of isolates of great significance.