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目的 研究有关生化因素对喜树碱（ＣＰＴ）诱导人鼻咽癌细胞凋亡的影响。方法 在ＣＰＴ诱导人鼻咽癌ＣＮＥ－２Ｚ 细胞凋亡的基础上，采用流式细胞仪及二苯胺反应等方法，观察了多胺、Ｃａ２＋、Ｍｇ２十依赖性内切酶活性抑制剂及ＰＡＲＰ活性抑 制剂对ＤＮＡ片段化形成的影响。结果 多胺可明显抑制ＣＰＴ诱导的ＣＮＥ－２Ｚ细胞凋亡，使片段化ＤＮＡ明显降低，Ｐ＜０．０５。内 切酶及ＰＡＲＰ活性抑制剂与ＣＰＴ共同作用ＣＮＥ－２Ｚ细胞后测得片段化ＤＮＡ与单用ＣＰＴ相比无显著性差异，Ｐ＞０．０５。结论 喜树碱诱导的 ＣＮＥ－２Ｚ细胞凋亡可能与一种非 Ｃａ２＋、Ｍｇ２＋依赖性内切酶活性有关，且受多胺的调节。
Objective To investigate the effects of biochemical factors on the apoptosis of human nasopharyngeal carcinoma cells induced by CPT. Methods CPT induced CNE-2Z cell apoptosis in human nasopharyngeal carcinoma based on the use of flow cytometry and diphenylamine reaction and other methods were observed Polyamine, Ca2, Mg2-dependent endonuclease activity inhibitor and PARP activity Effect of inhibitors on DNA fragmentation. Results Polyamines significantly inhibited the apoptosis of CNE-2Z cells induced by CPT and markedly reduced the fragmented DNA (P <0.05). Endonuclease and PARP inhibitor combined with CPT in CNE-2Z cells measured fragmented DNA and CPT alone was no significant difference, P> 0.05. CONCLUSION Camptothecin-induced apoptosis of CNE-2Z cells may be related to a non-Ca2 +, Mg2 + -dependent endonuclease activity and is regulated by polyamines.