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近年来,对于肺泡巨噬细胞在矽肺发病中的作用进行了大量的研究,这些研究推进了对矽肺发病的认识,为矽肺防治研究提供了理论基础。本文用电子显微镜观察了石英、二氧化钛对体外培养的肺泡巨噬细胞超微结构的影响,以了解其对细胞器的损伤作用;同时用氚标记亮氨酸掺入巨噬细胞的方法,观察了石英、石棉、石墨、二氧化钛粉尘对细胞合成蛋白功能的影响。 材料与方法 1.肺泡巨噬细胞(PAM)在无菌操作下,以肺灌洗法取健康雄性豚鼠PAM,用RPMI—1640培养液以贴壁培养方法提纯PAM,加不同浓度的石英、二氧化钛粉尘
In recent years, a great deal of research has been done on the role of alveolar macrophages in the pathogenesis of silicosis. These studies have promoted the understanding of silicosis and provided a theoretical basis for the study on the prevention and treatment of silicosis. In this paper, the effects of quartz and titanium dioxide on the ultrastructure of alveolar macrophages cultured in vitro were observed by electron microscope to understand the damage of organelles. Meanwhile, the incorporation of tritium and leucine into macrophages was used to observe the effects of quartz and titanium dioxide on the ultrastructure of alveolar macrophages. , Asbestos, graphite, titanium dioxide dust on cell protein synthesis function. Materials and methods 1. Alveolar macrophages (PAM) Aseptic male lungs were treated with PAM by pulmonary lavage. PAMs were purified by adherent culture with RPMI-1640 medium. Different concentrations of quartz, titanium dioxide dust