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用细胞学和统计学方法研究了外源Ca~(2+)对烟草(Nicotiana tabacum L.)离体花粉管生长和生殖核分裂的影响。正常培养条件下,花粉管群体内的生殖核分裂率大致呈对数增长,10～18h为其分裂高峰期。所用Ca~(2+)浓度中以10~(-3)mol/L最适于花粉管生长,与之相比,其它浓度随时间延长愈益明显地表现出抑制效应。生殖核分裂则以10~(-2)与10~(-3)mol/L较为适宜,且10~(-2)mol/L可相对提前分裂高峰。在含10~(-3)mol/L Ca~(2+)培养基中培养10h后用不同方法处理,发现高钙抑制花粉管生长,尤以10~(-1)mol/L Ca~(2+)抑制最强烈,导致花粉管顶端壁加厚及生殖核的无丝分裂。而10~(-2)mol/L Ca~(2+)在处理早期(10～12h)促进生殖核分裂。EGTA处理则同时抑制花粉管生长和生殖核分裂。
Cytological and statistical methods were used to study the effects of exogenous Ca ~ (2+) on pollen tube growth and reproductive mitosis in tobacco (Nicotiana tabacum L.). Under normal culture conditions, the population of pollen tube in the population reproductive mitosis rate of logarithmic growth, 10 ~ 18h for the peak of its division. Compared with the concentration of Ca ~ (2+), the optimum concentration of 10 ~ (-3) mol / L for pollen tube growth, compared with other concentrations of more and more obvious inhibitory effect. Reproductive nuclear fission was more suitable for 10 -2 and 10 -3 mol / L, and 10 -2 mol / L for early cleavage. After treated with 10 ~ (-3) mol / L Ca ~ (2+) medium for 10h, different treatments were applied and found that high calcium inhibited the growth of pollen tube, especially 10 ~ (-1) mol / L Ca ~ 2+) is most strongly inhibited, resulting in thickening of the top wall of the pollen tube and atrophy of the reproductive nucleus. However, 10 ~ (-2) mol / L Ca ~ (2+) promoted genital fission in the early stage of treatment (10 ~ 12h). EGTA treatment inhibited both pollen tube growth and reproductive nuclear fission.