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从‘章姬’草莓中克隆了一个MOT1家族成员的全长,测序发现其CDS序列与公布的森林草莓基因组序列一致,将其命名为FaMOT1。进化树分析表明Fa MOT1所编码的氨基酸与苹果和桃亲缘关系最近。荧光定量PCR结果表明,FaMOT1基因在草莓的根、茎、叶、花和10 d幼果中有不同程度的表达。对草莓幼苗喷施不同浓度的钼酸钠,结果发现,各处理叶片的钼浓度随着施钼量的增加而显著提高。2.4 mmol·L~(-1) Na2Mo O4处理显著诱导根部和叶片的FaMOT1的表达,处理2和4 h时4.8 mmol·L~(-1) Na_2MoO_4处理的茎部FaMOT1表达水平最高,处理6和12 h时2.4 mmol·L~(-1) Na_2MoO_4处理的茎部FaMOT1表达水平最高。2.4 mmol·L~(-1) Na_2MoO_4处理叶片的硝态氮浓度显著低于其它处理,氨态氮浓度显著高于其它处理;4.8 mmol·L~(-1)Na_2MoO_4处理根部和叶片的氨态氮浓度最低。以上结果表明FaMOT1基因的表达受到钼的诱导和调节,合适浓度的钼处理有利于硝态氮的吸收和向氨态氮的转化以及氨态氮向有机氮的转化。
The full length of a MOT1 family member was cloned from ’Zhangji’ strawberry. Sequencing revealed that its CDS sequence was consistent with the published forest strawberry genomic sequence and named it FaMOT1. Phylogenetic tree analysis showed that the amino acids encoded by Fa MOT1 are closest to apple and peach. Fluorescence quantitative PCR results showed that FaMOT1 gene expressed differently in roots, stems, leaves, flowers and young fruits of strawberry. Strawberry seedlings sprayed with different concentrations of sodium molybdate, the results showed that the treatment of leaf molybdenum concentration with the amount of molybdenum increased significantly. 2.4 mmol·L -1 Na2Mo O4 significantly induced the expression of FaMOT1 in roots and leaves. The expression of FaMOT1 in the roots treated with 4.8 mmol·L -1 Na_2MoO_4 was highest at 2 and 4 h, At 12 h, the expression level of FaMOT1 in stems treated with 2.4 mmol·L -1 Na 2 MoO 4 was the highest. The concentration of nitrate nitrogen in leaves treated with 2.4 mmol·L -1 Na 2 MoO 4 was significantly lower than that of other treatments, and the concentration of ammonia nitrogen was significantly higher than that of other treatments. The ammonia content of roots and leaves treated with 4.8 mmol·L -1 Na 2 MoO 4 The lowest nitrogen concentration. The above results indicated that the expression of FaMOT1 gene was induced and regulated by molybdenum. The molybdenum treatment at a suitable concentration was beneficial to the absorption of nitrate nitrogen, the conversion to ammonia nitrogen and the conversion of ammonia nitrogen to organic nitrogen.