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目的：确证新生牛促肝细胞生长因子（ Ｈ Ｇ Ｆ）的理化性质及生物学特性。方法：采用高效液相 色谱法（ Ｈ Ｐ Ｌ Ｃ）、 Ｓ Ｄ Ｓ聚丙烯酰胺凝胶电泳（ Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ）、紫外扫描、同位素３ Ｈ胸苷掺入法（３ Ｈ Ｔｄ Ｒ）测定 Ｈ Ｇ Ｆ的理化性质及生物学特性。结果： Ｈ Ｇ Ｆ主要为小分子肽，具有刺激损伤肝细胞 Ｄ Ｎ Ａ 合成，促进肝细胞再生的功能。结论： Ｈ Ｇ Ｆ 具有良好的肝脏保护与治疗作用。
Objective: To confirm the physical and chemical properties and biological characteristics of neonatal bovine hepatocyte growth factor (HG F). Methods: High-performance liquid chromatography (HPLC), SDS-polyacrylamide gel electrophoresis (SDS-PAGE), UV scanning and isotope 3H-thymidine incorporation Td R) was used to determine the physico-chemical properties and biological properties of H G F. Results: H G F was mainly composed of small peptides with the function of stimulating the synthesis of D N A in hepatocytes and promoting the regeneration of hepatocytes. Conclusion: H G F has good liver protection and therapeutic effect.