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血清甲状腺素(Tetfaiodothyronine;Thyro xine,简称T_4)RIA含量测定是目前临床上常用于诊断、鉴别诊断“甲亢”或“甲减”症,甲亢症及甲状腺肿瘤病人手术前的准备、治疗效果观察、随访复查、研究探讨甲状腺疾病以及甲状腺激素与其他疾病关系的指标之一,是对甲状腺功能判断最基本的筛选试验。 一、T_4放射免疫测定的基本原理:已知适当浓度的T_4特异性抗体与血清T_4和I-T_4之间竞争地结合,结合到抗体上的I—T_4部分与未标记T_4的浓度呈反比关系,利用分离剂将分离结合标记抗原和游离标记抗原,测量沉淀部分的放射性,根据结合率标准曲线,样品中T_4浓度就能查知。 二、参考正常值:上海放射免疫分析技术研究所生产的血清T_4 RIA试剂盒,正常值为45～130ng/ml。根据文献报道,不同年龄、地区及实验室,参考正常值有如下几种情况。 (一)新生儿出生后三天内的血清:1天18.6±4.39μg％(范围11～28μg％);2天15.0±2.80μg％(范囱9～20μg％);3天14.9±4.33μ％(范围8.4～24μg％)。
Thyroxine (Thyroxine, referred to as T_4) RIA assay is currently clinically used in the diagnosis, differential diagnosis of “hyperthyroidism” or “hypothyroidism”, hyperthyroidism and thyroid tumors in patients with preoperative preparation, the treatment effect, Follow-up review, research to explore thyroid disease and thyroid hormone and other diseases, one of the indicators, thyroid function is to determine the most basic screening test. First, the basic principle of radioimmunoassay T_4: Known appropriate concentrations of T_4-specific antibodies and competitive binding between serum T_4 and I-T_4, I-T_4 part of the antibody bound to the concentration of unlabeled T_4 was inversely proportional , The separation agent will be used to separate the labeled antigen and the labeled antigen, the precipitation part of the radioactivity measured, according to the binding rate of the standard curve, the sample can be detected T_4 concentration. Second, the reference normal: Shanghai Radioimmunoassay Institute of Technology serum T_4 RIA kit, the normal value of 45 ~ 130ng / ml. According to the literature, different ages, regions and laboratories, reference to the normal value of the following situations. (A) Serum within three days after birth of a newborn: 18.6 ± 4.39 μg% (range 11-28 μg%) for one day; 15.0 ± 2.80 μg% (vancomycin 9-20 μg%) for 2 days; 14.9 ± 4.33 μ% (Range 8.4 to 24 μg%).