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目的 :观察钙超载大鼠心肌肌浆网 (SR )功能的变化。方法 :腹腔注射维生素D3和nicotine灌胃复制大鼠心肌钙超载模型。差速离心制备心肌肌浆网 ,微孔过滤技术测定SR的钙释放与钙摄取 ,放射配基法测定SRryanodine受体结合能力。结果 :钙超载大鼠心肌钙含量比对照组高 32 4% (P <0 0 1) ,心肌SR钙摄取和钙释放分别低 6 4%和 40 % (P <0 0 1)。心肌SRCa2 + -ATP酶活性低 6 5 % (P <0 0 1)。SR特异 [3H]-ryanodine结合的最大值Bmax低 5 1% (P <0 0 1)。结论 :钙超载大鼠心肌SR功能下降。
Objective: To observe the changes of cardiac sarcoplasmic reticulum (SR) function in rats with calcium overload. Methods: Intraperitoneal injection of vitamin D3 and nicotine intragastrically in rat cardiac calcium overload model. Cardiac sarcoplasmic reticulum was prepared by differential centrifugation. Calcium release and calcium uptake by SR were measured by microporous filtration. Radioligand was used to determine SRryanodine receptor binding ability. Results: Compared with the control group, the calcium content in calcium-overload rats was 32 4% (P <0.01), and the SR calcium absorption and calcium release in rats were lower by 6.4% and 40% (P <0.01), respectively. Myocardial SRCa2 + -ATP activity was 65% lower (P <0.01). The maximum Bmax for SR-specific [3H] -ryanodine binding was 51% lower (P <0.01). Conclusion: The cardiac function of calcium overload rats decreased.