论文部分内容阅读
将双十二烷基二甲基溴化铵 ( DDAB) -粘土 ( Clay)复合物的水分散系与肌红蛋白 ( Mb)水溶液的混合物涂布到热解石墨 ( PG)电极表面 ,可制得 Mb-DDAB-Clay薄膜电极 .在 p H5.5的缓冲溶液中 ,该薄膜电极在-0 .2 5V( vs.SCE)处有一对可逆的循环伏安还原氧化峰 ,为 Mb血红素辅基 Fe( ) /Fe( )电对的特征峰 .在 DDAB-Clay薄膜的微环境中 ,Mb与 PG电极之间的电子传递得到极大促进 ,并显示了很好的稳定性 .Soret吸收带的位置表明 ,在适中的 p H范围内 ,Mb在薄膜中保持了其原始构象 .X射线衍射实验结果表明 ,Mb的嵌入并未对薄膜的有序多层结构有很大影响 .在 DDAB-Clay薄膜环境中 ,Mb血红素 Fe( ) /Fe( )电对的式量电位在 p H4 .5～ 1 1 .0范围内与溶液 p H值成线性关系 ,表明 Mb的电化学还原很可能是一个质子伴随一个电子的电极过程 .Mb-DDAB-Clay薄膜可以用于催化还原溶解氧和三氯乙酸
A mixture of an aqueous dispersion of DDAB-Clay complex and an aqueous solution of myoglobin (Mb) was applied to the surface of a pyrolytic graphite (PG) electrode to prepare In the buffer solution of p H5.5, the membrane electrode has a pair of reversible cyclic voltammetry reduction oxidation peaks at -0.2 V (vs.SCE) for the Mb heme complement The characteristic peaks of Fe () / Fe () group are shown in Figure 2. The electron transfer between Mb and PG electrode is greatly promoted and shows good stability in the DDAB-Clay thin film microenvironment. Shows that in the moderate range of p H, Mb retains its original conformation in the film.The results of X-ray diffraction show that the embedding of Mb has no significant effect on the ordered multi-layer structure of the film.In DDAB- In the Clay film environment, the mass potential of the Mb heme Fe () / Fe () pairs is linear with the p H value of the solution over the range of p H4 .5 to 1.1 0, indicating that the electrochemical reduction of Mb is very likely Is a proton accompanied by an electronic electrode process .Mb-DDAB-Clay film can be used for catalytic reduction of dissolved oxygen and trichloroacetic acid