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目的:探讨坎离颗粒对腹主动脉缩窄所致心衰大鼠心肌间质重构的影响及可能作用机制。方法:腹主动脉缩窄法制备大鼠心衰模型,分为模型组、中药组(坎离颗粒)和阳性对照组(卡托普利),另设假手术组,每组15只;造模12周后各治疗组分别给予坎离颗粒水溶液12g.kg-1.d-1和卡托普利混悬液3.375mg.kg-1.d-1,假手术组和模型组给予等量双蒸水。干预24周后处死大鼠,检测左室质量指数(LVMI)、左室腔内径(LVD)、左室后壁厚度(LVPWT);苦味酸天狼猩红染色测量胶原容积分数(CVF);免疫组化法测量心肌左室心肌基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶抑制剂-1(TIMP-1)、转化生长因子-β1(TGF-β1)的含量。结果:坎离颗粒可降低CVF、心脏指数(P<0.05)和LVMI(P<0.01),升高I/Ⅲ型胶原比值(P<0.05)、LVD(P<0.01);纠正心衰大鼠心肌的TGF-β1(P<0.01)、MMP-9(P<0.01)、TIMP-1(P<0.05)异常表达,部分指标优于对照组。结论:坎离颗粒可能通过减少心肌MMP-9、TGF-β1和增加TIMP-1的含量,而减轻心肌胶原网络的破坏及反应性胶原的过度沉积,从而改善心肌间质重构,改善心脏功能。
Objective: To investigate the effect of Kanli Granule on cardiac interstitial remodeling in heart failure rats with abdominal aorta constriction and its possible mechanism. Methods: A rat model of heart failure was established by abdominal aorta constriction. The model group was divided into model group, Chinese medicine group (Kanli particles) and positive control group (Captopril) After 12 weeks of treatment, 12 g · kg-1.d-1 aqueous solution of Kanli granule and 3.375 mg · kg-1.d-1 of captopril suspension were respectively given to the treatment groups, and the sham operation group and the model group were given the same amount Double distilled water. The rats were sacrificed 24 weeks after the intervention, and the left ventricular mass index (LVMI), left ventricular cavity diameter (LVD) and left ventricular posterior wall thickness (LVPWT) were measured. The collagen volume fraction (CVF) Methods The contents of MMP-9, TIMP-1 and TGF-β1 in myocardial left ventricular myocardium were measured. Results: KANGYI granules could reduce CVF, heart index (P <0.05) and LVMI (P <0.01), increase the ratio of type I / III collagen (P <0.05), LVD The levels of TGF-β1 (P <0.01), MMP-9 (P <0.01), and TIMP-1 (P <0.05) in myocardium were significantly higher than those in control group. CONCLUSION: KANGYI granule can reduce myocardial collagen network destruction and over-deposition of reactive collagen by decreasing MMP-9, TGF-β1 and increasing TIMP-1 in myocardium so as to improve myocardial interstitial remodeling and improve cardiac function .